rabbit anti mt2  (Novus Biologicals)

Journal: Animals : an Open Access Journal from MDPI

Article Title: Melatonin in Male Dromedary Camel ( Camelus dromedarius ) Seminal Plasma and Its Specific MT1 and MT2 Receptors on Sperm Membranes

doi: 10.3390/ani15010083

Figure Lengend Snippet: Distribution of MT2 melatonin receptors in camel spermatozoa, evaluated by the indirect immunoassay method. Immunostaining in head (H), acrosome (A), post-acrosome (PA), neck (N), tail (T) and apical edge (AE) was evidenced. Magnification 1000×. MT2 receptors ( A , C , E , G ) and bright field ( B , D , F , H ) are shown.

Article Snippet: After the blocking of non-specific sites on the membrane with SuperBlock Blocking Buffer (ThermoFisher Scientific, Waltham, MA, USA) for 10 min with shaking, the melatonin receptors were immunodetected by incubating for 1 h at room temperature with the primary antibody Mel-1A-R rabbit polyclonal antibody against the MT1 receptor (GeneTex Inc., Irvine, CA, USA; Cat# GTX100003, RRID: AB_1241048) or rabbit polyclonal antibody against the MT2 receptor (Acris Antibodies, GmbH, Herford, Germany; Cat# AP01322PU-N, RRID: AB_1619198), both diluted 1:500 in the Primary Antibody Diluent (ThermoFisher Scientific, Waltham, MA, USA).

Techniques: Immunostaining

Journal: Animals : an Open Access Journal from MDPI

Article Title: Melatonin in Male Dromedary Camel ( Camelus dromedarius ) Seminal Plasma and Its Specific MT1 and MT2 Receptors on Sperm Membranes

doi: 10.3390/ani15010083

Figure Lengend Snippet: Indirect immunofluorescence controls. Samples were incubated with only the MT1 ( A ) or MT2 ( C ) primary or secondary antibody ( E ) and ( G ) for Alexa Fluor 594 anti-mouse antibody and Alexa Fluor 488 anti-rabbit antibody, respectively). Magnification 1000×. Fluorescence after 30 s exposition ( A , C , E , G ) and bright field ( B , D , F , H ) are shown.

Article Snippet: After the blocking of non-specific sites on the membrane with SuperBlock Blocking Buffer (ThermoFisher Scientific, Waltham, MA, USA) for 10 min with shaking, the melatonin receptors were immunodetected by incubating for 1 h at room temperature with the primary antibody Mel-1A-R rabbit polyclonal antibody against the MT1 receptor (GeneTex Inc., Irvine, CA, USA; Cat# GTX100003, RRID: AB_1241048) or rabbit polyclonal antibody against the MT2 receptor (Acris Antibodies, GmbH, Herford, Germany; Cat# AP01322PU-N, RRID: AB_1619198), both diluted 1:500 in the Primary Antibody Diluent (ThermoFisher Scientific, Waltham, MA, USA).

Techniques: Immunofluorescence, Incubation, Fluorescence

Journal: Animals : an Open Access Journal from MDPI

Article Title: Melatonin in Male Dromedary Camel ( Camelus dromedarius ) Seminal Plasma and Its Specific MT1 and MT2 Receptors on Sperm Membranes

doi: 10.3390/ani15010083

Figure Lengend Snippet: Comparison among age classes of males at different melatonin receptors localizations (MT1 ( A ) and MT2 ( B )) in acrosome (A), post-acrosome (PA), head (H), neck (N), tail (T), cytoplasmic droplet (CD) and apical edge (AE). Values are shown as percentage of localization of n = 439 spermatozoa. * indicates p < 0.05.

Article Snippet: After the blocking of non-specific sites on the membrane with SuperBlock Blocking Buffer (ThermoFisher Scientific, Waltham, MA, USA) for 10 min with shaking, the melatonin receptors were immunodetected by incubating for 1 h at room temperature with the primary antibody Mel-1A-R rabbit polyclonal antibody against the MT1 receptor (GeneTex Inc., Irvine, CA, USA; Cat# GTX100003, RRID: AB_1241048) or rabbit polyclonal antibody against the MT2 receptor (Acris Antibodies, GmbH, Herford, Germany; Cat# AP01322PU-N, RRID: AB_1619198), both diluted 1:500 in the Primary Antibody Diluent (ThermoFisher Scientific, Waltham, MA, USA).

Techniques: Comparison

Journal: Animals : an Open Access Journal from MDPI

Article Title: Melatonin in Male Dromedary Camel ( Camelus dromedarius ) Seminal Plasma and Its Specific MT1 and MT2 Receptors on Sperm Membranes

doi: 10.3390/ani15010083

Figure Lengend Snippet: Representative Western blot images showing the presence of MT1 ( A ) and MT2 ( B ) melatonin receptors in protein extracts from camel spermatozoa (M: Molecular weight marker (kDa), C: Camel sperm proteins, (+): Positive ram control).

Article Snippet: After the blocking of non-specific sites on the membrane with SuperBlock Blocking Buffer (ThermoFisher Scientific, Waltham, MA, USA) for 10 min with shaking, the melatonin receptors were immunodetected by incubating for 1 h at room temperature with the primary antibody Mel-1A-R rabbit polyclonal antibody against the MT1 receptor (GeneTex Inc., Irvine, CA, USA; Cat# GTX100003, RRID: AB_1241048) or rabbit polyclonal antibody against the MT2 receptor (Acris Antibodies, GmbH, Herford, Germany; Cat# AP01322PU-N, RRID: AB_1619198), both diluted 1:500 in the Primary Antibody Diluent (ThermoFisher Scientific, Waltham, MA, USA).

Techniques: Western Blot, Molecular Weight, Marker, Control

Journal: Animals : an Open Access Journal from MDPI

Article Title: Melatonin in Male Dromedary Camel ( Camelus dromedarius ) Seminal Plasma and Its Specific MT1 and MT2 Receptors on Sperm Membranes

doi: 10.3390/ani15010083

Figure Lengend Snippet: Distribution of MT2 melatonin receptors in camel spermatozoa, evaluated by the indirect immunoassay method. Immunostaining in head (H), acrosome (A), post-acrosome (PA), neck (N), tail (T) and apical edge (AE) was evidenced. Magnification 1000×. MT2 receptors ( A , C , E , G ) and bright field ( B , D , F , H ) are shown.

Article Snippet: Following three additional PBS washes, the spermatozoa were incubated with the primary antibody for melatonin receptor MT1 (MTNR1A mouse polyclonal antibody; Abnova, Taipei, Taiwan; Cat# H00004543-A01, RRID: AB_462681) diluted 1:10 in PBS with 1% BSA, or the primary antibody for melatonin receptor MT2 (MTNR1B Rabbit Polyclonal Antibody, Acris Antibodies GmbH, Herford, Germany; Cat# AP01322PU-N, RRID: AB_1619198) diluted 1:20 in PBS with 1% BSA overnight at 4 °C in a wet chamber.

Techniques: Immunostaining

Journal: Animals : an Open Access Journal from MDPI

Article Title: Melatonin in Male Dromedary Camel ( Camelus dromedarius ) Seminal Plasma and Its Specific MT1 and MT2 Receptors on Sperm Membranes

doi: 10.3390/ani15010083

Figure Lengend Snippet: Indirect immunofluorescence controls. Samples were incubated with only the MT1 ( A ) or MT2 ( C ) primary or secondary antibody ( E ) and ( G ) for Alexa Fluor 594 anti-mouse antibody and Alexa Fluor 488 anti-rabbit antibody, respectively). Magnification 1000×. Fluorescence after 30 s exposition ( A , C , E , G ) and bright field ( B , D , F , H ) are shown.

Article Snippet: Following three additional PBS washes, the spermatozoa were incubated with the primary antibody for melatonin receptor MT1 (MTNR1A mouse polyclonal antibody; Abnova, Taipei, Taiwan; Cat# H00004543-A01, RRID: AB_462681) diluted 1:10 in PBS with 1% BSA, or the primary antibody for melatonin receptor MT2 (MTNR1B Rabbit Polyclonal Antibody, Acris Antibodies GmbH, Herford, Germany; Cat# AP01322PU-N, RRID: AB_1619198) diluted 1:20 in PBS with 1% BSA overnight at 4 °C in a wet chamber.

Techniques: Immunofluorescence, Incubation, Fluorescence

Journal: Animals : an Open Access Journal from MDPI

Article Title: Melatonin in Male Dromedary Camel ( Camelus dromedarius ) Seminal Plasma and Its Specific MT1 and MT2 Receptors on Sperm Membranes

doi: 10.3390/ani15010083

Figure Lengend Snippet: Comparison among age classes of males at different melatonin receptors localizations (MT1 ( A ) and MT2 ( B )) in acrosome (A), post-acrosome (PA), head (H), neck (N), tail (T), cytoplasmic droplet (CD) and apical edge (AE). Values are shown as percentage of localization of n = 439 spermatozoa. * indicates p < 0.05.

Article Snippet: Following three additional PBS washes, the spermatozoa were incubated with the primary antibody for melatonin receptor MT1 (MTNR1A mouse polyclonal antibody; Abnova, Taipei, Taiwan; Cat# H00004543-A01, RRID: AB_462681) diluted 1:10 in PBS with 1% BSA, or the primary antibody for melatonin receptor MT2 (MTNR1B Rabbit Polyclonal Antibody, Acris Antibodies GmbH, Herford, Germany; Cat# AP01322PU-N, RRID: AB_1619198) diluted 1:20 in PBS with 1% BSA overnight at 4 °C in a wet chamber.

Techniques: Comparison

Journal: Animals : an Open Access Journal from MDPI

Article Title: Melatonin in Male Dromedary Camel ( Camelus dromedarius ) Seminal Plasma and Its Specific MT1 and MT2 Receptors on Sperm Membranes

doi: 10.3390/ani15010083

Figure Lengend Snippet: Representative Western blot images showing the presence of MT1 ( A ) and MT2 ( B ) melatonin receptors in protein extracts from camel spermatozoa (M: Molecular weight marker (kDa), C: Camel sperm proteins, (+): Positive ram control).

Article Snippet: Following three additional PBS washes, the spermatozoa were incubated with the primary antibody for melatonin receptor MT1 (MTNR1A mouse polyclonal antibody; Abnova, Taipei, Taiwan; Cat# H00004543-A01, RRID: AB_462681) diluted 1:10 in PBS with 1% BSA, or the primary antibody for melatonin receptor MT2 (MTNR1B Rabbit Polyclonal Antibody, Acris Antibodies GmbH, Herford, Germany; Cat# AP01322PU-N, RRID: AB_1619198) diluted 1:20 in PBS with 1% BSA overnight at 4 °C in a wet chamber.

Techniques: Western Blot, Molecular Weight, Marker, Control